Difference between revisions of "Part:BBa K606049"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K606049 short</partinfo> | <partinfo>BBa_K606049 short</partinfo> | ||
| − | This sequence is the | + | This sequence is the AttL recombination sequence from the lambda phage. It has been synthetized de novo by GeneArt and submitted to the registry once clones into pSB1C3. |
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| + | The idea is that this part can be used in combination with the part [https://parts.igem.org/Part:BBa_K606056 K606056]. When expressing the Xis protein, the stop can be excised, and the transcription recovered. | ||
| + | [[Image:Excision_xis-reporter.jpg|thumb|center]] | ||
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| + | The sequence of this part is not exactly the one you can find for the similar parts in the registry. We took it from cross-validating several articles from the litterature. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 21:52, 25 October 2011
Lambda phage attL recombination site
This sequence is the AttL recombination sequence from the lambda phage. It has been synthetized de novo by GeneArt and submitted to the registry once clones into pSB1C3.
The idea is that this part can be used in combination with the part K606056. When expressing the Xis protein, the stop can be excised, and the transcription recovered.
The sequence of this part is not exactly the one you can find for the similar parts in the registry. We took it from cross-validating several articles from the litterature.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]