Difference between revisions of "Part:BBa K606061"
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===References===
 
===References===
 
<biblio>
 
<biblio>
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#1 pmid=9669336  Mol Gen Genet. 1998 Jun;258(5):538-45.
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#1 pmid=9669336  [Mol Gen Genet. 1998 Jun;258(5):538-45]
 
</biblio>
 
</biblio>

Latest revision as of 20:53, 25 October 2011

SpoVG Ribosome Binding Site (RBS) for B. subtilis

SpoVG Ribosome Binding Site (RBS) for B. subtilis.

This is a strong ribosome binding site from suibtilis. This part is a copy of the part from Imperial college 2008, BBa_K143021, but we have synthetized it de-novo, using primer matching technique, and submitted it to the registry.

This parts also works fine in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This part can be use to express proteins in both B. subtilis and E. coli, and leads to a strong expression of the protein. The original sequence come from the reference following reference [1]

References

<biblio>

  1. 1 pmid=9669336 [Mol Gen Genet. 1998 Jun;258(5):538-45]

</biblio>