Difference between revisions of "Part:BBa K606024:Experience"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
−
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+
==Characterization: Biobricked YFP:TetR==
−
how you used this part and how it worked out.
+
 
 +
'''Parts construction''':<br>
 +
<html>
 +
Origins of YFP:TetR are from pFX234 of D. Lane (Toulouse 2 University).
 +
<center><img src="https://static.igem.org/mediawiki/2011/c/cb/YFPtetR10.jpg">
 +
<p>Cloning plan of YFP:TetR construction</center></p>
 +
</html>
 +
<br><br>We characterize it with TetO Array from pDAG479 plasmid of F-X Barre and D. Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004).<br><br>
 +
 
 +
'''Microscopy of double transformed pDAG470 / Biobricked YFP:TetR in <i>E. Coli</i>:'''
 +
 
 +
<p>To characterize this part properly, we took pictures of different strains containing YFP:TetR alone and both TetO array (from pDAG479, D.Lane) and expression YFP:TetR system (BBa_K606027).</p>
 +
 
 +
<center>
 +
{| border="1" class="wikitable" style="text-align: center;" align="center"
 +
|+ Biobricked YFP:TetR only
 +
|-
 +
|[[Image:yfptetrbb_trans.jpg|350px|thumb|center|Biobricked expression YFP:tetR system in E. coli.]]
 +
|[[Image:yfptetrbb_Fluo2.jpg|350px|thumb|center|Biobricked expression YFP:tetR system in E. coli.]]
 +
|}
 +
{| border="1" class="wikitable" style="text-align: center;" align="center"
 +
|+ Biobricked YFP:TetR and TetO Array
 +
|-
 +
|[[Image:yfptetrbb_teto_trans.jpg|350px|thumb|center|Biobricked expression YFP:tetR system and TetO Array from D. Lane in E. coli.]]
 +
|[[Image:yfptetrbb_teto__Fluo2.jpg|350px|thumb|center|Biobricked expression YFP:tetR system and TetO Array from D. Lane in E. coli.]]
 +
|}
 +
</center>
 +
 
 +
The TetR-YFP construct which (emitter part) occasionally shows gross aggregated YFP. <br>
 +
After observing the cells carrying both the TetO and YFP:TetR constructs, we can obviously distinguish glowing dots in some cells. They reflect the behavior we expected.
 +
 
  
−
===Applications of BBa_K606024===
+
More information on : TetO Array characterization (<partinfo>BBa_K606026</partinfo>) and iGEM Paris Bettencourt 2011 wiki [http://2011.igem.org/Team:Paris_Bettencourt/Experiments/YFP_TetR_diffusion]
  
 
===User Reviews===
 
===User Reviews===

Revision as of 16:27, 24 October 2011

Characterization: Biobricked YFP:TetR

Parts construction:
Origins of YFP:TetR are from pFX234 of D. Lane (Toulouse 2 University).

Cloning plan of YFP:TetR construction



We characterize it with TetO Array from pDAG479 plasmid of F-X Barre and D. Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004).

Microscopy of double transformed pDAG470 / Biobricked YFP:TetR in E. Coli:

To characterize this part properly, we took pictures of different strains containing YFP:TetR alone and both TetO array (from pDAG479, D.Lane) and expression YFP:TetR system (BBa_K606027).

Biobricked YFP:TetR only
Biobricked expression YFP:tetR system in E. coli.
Biobricked expression YFP:tetR system in E. coli.
Biobricked YFP:TetR and TetO Array
Biobricked expression YFP:tetR system and TetO Array from D. Lane in E. coli.
Biobricked expression YFP:tetR system and TetO Array from D. Lane in E. coli.

The TetR-YFP construct which (emitter part) occasionally shows gross aggregated YFP.
After observing the cells carrying both the TetO and YFP:TetR constructs, we can obviously distinguish glowing dots in some cells. They reflect the behavior we expected.


More information on : TetO Array characterization (BBa_K606026) and iGEM Paris Bettencourt 2011 wiki [http://2011.igem.org/Team:Paris_Bettencourt/Experiments/YFP_TetR_diffusion]

User Reviews

UNIQ56a51eac2ca40804-partinfo-00000002-QINU UNIQ56a51eac2ca40804-partinfo-00000003-QINU