Difference between revisions of "Part:BBa K606065:Design"
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===Design Notes=== | ===Design Notes=== | ||
This sequence is the pure tRNA without the side sequences. This part was initially designed for B.subtillis but it also works in E.coli (look at our experience page BBa_K606034). | This sequence is the pure tRNA without the side sequences. This part was initially designed for B.subtillis but it also works in E.coli (look at our experience page BBa_K606034). | ||
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===Source=== | ===Source=== | ||
Revision as of 17:38, 23 October 2011
IPTG inducible tRNA Amber emitter with RFP monitor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 678
Illegal AgeI site found at 790 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence is the pure tRNA without the side sequences. This part was initially designed for B.subtillis but it also works in E.coli (look at our experience page BBa_K606034).
Source
Synthetize de novo by matching primers and cloned from the tRNA cluster of B. Subtilis genome of strain 168