Difference between revisions of "Part:BBa J176013"
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===Characterization=== | ===Characterization=== | ||
| − | <b>VP64 activity is sensitive to promoter proximity</b> | + | <b>VP64 activity is sensitive to promoter proximity.</b><br> |
In our hands, VP64 activity is diminished when it is targeted too close to a promoter. When fused to the Gal4 DNA binding domain and targeted to Gal (UAS) DNA elements that are assembled immediately adjacent to the HSVTkTATA minimal promoter ([part:BBa_J176011]), we observe weak activation, and perhaps repression, of a fluorescent reporter gene. In contrast, when a small spacer is inserted between the Gal4 binding sites and the promoter, reporter gene activity greatly increases. | In our hands, VP64 activity is diminished when it is targeted too close to a promoter. When fused to the Gal4 DNA binding domain and targeted to Gal (UAS) DNA elements that are assembled immediately adjacent to the HSVTkTATA minimal promoter ([part:BBa_J176011]), we observe weak activation, and perhaps repression, of a fluorescent reporter gene. In contrast, when a small spacer is inserted between the Gal4 binding sites and the promoter, reporter gene activity greatly increases. | ||
Revision as of 02:18, 16 October 2011
VP64
Tetrameric VP16 transc. activator domain
Usage and Biology
VP64 is a transcriptional activator composed of four tandem copies of VP16 (viral protein 16). When fused to another protein domain that can interact directly with a gene (e.g., a DNA binding domain), VP64 acts as a strong transcriptional activator.
Characterization
VP64 activity is sensitive to promoter proximity.
In our hands, VP64 activity is diminished when it is targeted too close to a promoter. When fused to the Gal4 DNA binding domain and targeted to Gal (UAS) DNA elements that are assembled immediately adjacent to the HSVTkTATA minimal promoter ([part:BBa_J176011]), we observe weak activation, and perhaps repression, of a fluorescent reporter gene. In contrast, when a small spacer is inserted between the Gal4 binding sites and the promoter, reporter gene activity greatly increases.
(Data will be posted soon)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]