Difference between revisions of "Part:BBa K571005:Experience"
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BBa_K571005 must be cloned together with BBa_K571010 into the E.coli in order to be a complete system that can produce cellulase synthase. We also verified our plasmid by PCR method and the acsAB is seen.<br> | BBa_K571005 must be cloned together with BBa_K571010 into the E.coli in order to be a complete system that can produce cellulase synthase. We also verified our plasmid by PCR method and the acsAB is seen.<br> | ||
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BBa_K571005 with the BBa_K571010 cloned into the E coli are then cultured with OD=0.5, then we collect the protein produced every 2 hours starting from 0hr, to run the SDS-PAGE for checking the expression of acsAB. <br> | BBa_K571005 with the BBa_K571010 cloned into the E coli are then cultured with OD=0.5, then we collect the protein produced every 2 hours starting from 0hr, to run the SDS-PAGE for checking the expression of acsAB. <br> | ||
Latest revision as of 08:27, 15 October 2011
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Applications of BBa_K571005
BBa_K571005
BBa_K571005 must be cloned together with BBa_K571010 into the E.coli in order to be a complete system that can produce cellulase synthase. We also verified our plasmid by PCR method and the acsAB is seen.
BBa_K571005 with the BBa_K571010 cloned into the E coli are then cultured with OD=0.5, then we collect the protein produced every 2 hours starting from 0hr, to run the SDS-PAGE for checking the expression of acsAB.
Gluconacetobacter Hansenii with acs operon is the experiment positive control while normal E coli will be our negative control. From the band we obtained from SDS-PAGE, the figure shows that acsA and acsB protein is expressed.
The relative molecular mass of acsAB is about 168KDa. Data shows that the expression of cellulose synthase starts from 0hr and it’s even more than the positive control.
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