Difference between revisions of "Part:BBa K571010:Experience"

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<font=bold>BBa_K571010 <br><br></font>
 
<font=bold>BBa_K571010 <br><br></font>
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BBa_K571010 must be cloned together with BBa_K571005 into the E.coli in order to be a complete system that can produce cellulase synthase. It also has Cmcax and Ccp genes which can enhance the productivity of cellulose via cellulase synthase. We also verified our plasmid by PCR method and the acsCD and Cmcax and Ccp genes are seen.<br><br>
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<img src="https://static.igem.org/mediawiki/parts/9/99/1010.PNG" hieght="500px"><br>
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BBa_K571010 must be cloned together with BBa_K571005 into the E.coli in order to be a complete system that can produce cellulase synthase. It also has Cmcax and Ccp genes which can enhance the productivity of cellulose via cellulase synthase. We also verified our plasmid by PCR method and the acsCD and Cmcax and Ccp genes are seen.<br><img src ="https://static.igem.org/mediawiki/parts/d/d3/Tu.PNG" hieght="300px"><br>
  
  
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BBa_K571010 with the BBa_K571005 cloned into the E coli are then cultured with OD=0.5, then we collect the protein produced every 2 hours starting from 0hr, to run the SDS-PAGE for checking the expression of acsCD. <br><br>
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BBa_K571010 with the BBa_K571005 cloned into the E coli are then cultured with OD=0.5, then we collect the protein produced every 2 hours starting from 0hr, to run the SDS-PAGE for checking the expression of acsCD.
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Gluconacetobacter Hansenii with acs operon is the experiment positive control while normal E coli will be our negative control. From the band we obtained from SDS-PAGE, the figure shows that acsC and acsD protein is expressed.<br>  
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Gluconacetobacter Hansenii with acs operon is the experiment positive control while normal E coli will be our negative control. From the band we obtained from SDS-PAGE, the figure shows that acsC and acsD protein is expressed.
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<br><img src="https://static.igem.org/mediawiki/parts/4/47/Sds.PNG" hieght= "800px"><br>
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<br><img src = "https://static.igem.org/mediawiki/parts/5/51/Chart.PNG" hieght ="800px"><br>
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The relative molecular mass of a acsC is about 138KDa acsD is about 17KDa. Data shows that the expression of cellulose synthase starts from 0hr and it’s even more than the positive control.
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Latest revision as of 08:02, 15 October 2011

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Applications of BBa_K571010

BBa_K571010


BBa_K571010 must be cloned together with BBa_K571005 into the E.coli in order to be a complete system that can produce cellulase synthase. It also has Cmcax and Ccp genes which can enhance the productivity of cellulose via cellulase synthase. We also verified our plasmid by PCR method and the acsCD and Cmcax and Ccp genes are seen.

BBa_K571010 with the BBa_K571005 cloned into the E coli are then cultured with OD=0.5, then we collect the protein produced every 2 hours starting from 0hr, to run the SDS-PAGE for checking the expression of acsCD. Gluconacetobacter Hansenii with acs operon is the experiment positive control while normal E coli will be our negative control. From the band we obtained from SDS-PAGE, the figure shows that acsC and acsD protein is expressed.



The relative molecular mass of a acsC is about 138KDa acsD is about 17KDa. Data shows that the expression of cellulose synthase starts from 0hr and it’s even more than the positive control.


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