Difference between revisions of "Part:BBa K559000:Experience"

 
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
how you used this part and how it worked out.
 
how you used this part and how it worked out.
 +
 +
===Characterizations of BBa_K559000===
 +
 +
'''Size Confirmation of the Biobrick'''
 +
 +
[[Image:Gel_BBa_K559000.png]]
 +
 +
The above figure shows the size check of our biobrick. Lane 1 represents 100 bp DNA ladder (NEB, cat. N3231L) , lane 3 represents our biobrick halorhodopsin gene. It matched the theoretical size of 640bp.
 +
 +
  
 
===Applications of BBa_K559000===
 
===Applications of BBa_K559000===
 +
We have used this biobrick as a basic unit to construct a functional unit <partinfo>BBa_K559010</partinfo>.
 +
 +
More characterizations on the regulated protein expression, chloride absorption condition and optimization were shown in <partinfo>BBa_K559010</partinfo>.
  
 
===User Reviews===
 
===User Reviews===
Line 17: Line 29:
 
Enter the review inofrmation here.
 
Enter the review inofrmation here.
 
|};
 
|};
 +
<!-- End of the user review template -->
 +
 +
 +
{|width='80%' style='border:1px solid gray'
 +
|-
 +
|width='10%'|
 +
<partinfo>BBa_K559000 AddReview 1</partinfo>
 +
<I>[[User: Jacky_Loo]]</I>
 +
|width='60%' valign='top'|
 +
 +
<partinfo>BBa_K559000</partinfo>, integrated into the halorhodopsin complete system generator <partinfo>BBa_K559010 </partinfo>, express Maxmized halorhodopsin membrane protein when supplemented with 0.1mM IPTG. Characterization of the IPTG addition to get maximum expression can be view in [https://parts.igem.org/Part:BBa_K559010:Experience]
 +
 +
|}
 +
 +
 
<!-- End of the user review template -->
 
<!-- End of the user review template -->
 
<!-- DON'T DELETE --><partinfo>BBa_K559000 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K559000 EndReviews</partinfo>

Latest revision as of 22:13, 14 October 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Characterizations of BBa_K559000

Size Confirmation of the Biobrick

Gel BBa K559000.png

The above figure shows the size check of our biobrick. Lane 1 represents 100 bp DNA ladder (NEB, cat. N3231L) , lane 3 represents our biobrick halorhodopsin gene. It matched the theoretical size of 640bp.


Applications of BBa_K559000

We have used this biobrick as a basic unit to construct a functional unit BBa_K559010.

More characterizations on the regulated protein expression, chloride absorption condition and optimization were shown in BBa_K559010.

User Reviews

UNIQ5fc34fbf05efcfb3-partinfo-00000002-QINU


•

User: Jacky_Loo

BBa_K559000, integrated into the halorhodopsin complete system generator BBa_K559010, express Maxmized halorhodopsin membrane protein when supplemented with 0.1mM IPTG. Characterization of the IPTG addition to get maximum expression can be view in [1]


UNIQ5fc34fbf05efcfb3-partinfo-00000006-QINU