Difference between revisions of "Part:BBa K624002"

 
 
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__NOTOC__
 
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<partinfo>BBa_K624002 short</partinfo>
 
<partinfo>BBa_K624002 short</partinfo>
  
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tetR and GFP under pT7 promoter control.
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TetR and GFP genes are both under pT7 promoter control. However, pT7 can only function under presence of associated regulatory protein; thus, for example, when we transform this construct into strain BL21 E. coli, which express associated regulator, both tetR and GFP will be expressed.In contrast, if we transform this construct into other strain, such as DH5alpha and S17-1,  both tetR and GFP genes will not be expressed. Due to that tetR and GFP are under the regulation of the same promoter, we can quantitize the tetR expression by detection intensity of GFP.
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Should be used in the T7 system to express the Gene
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GFP can help detecting tetR expression.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:52, 14 October 2011

pT7+RBS+tetR+RBS+GFP+Ter

TetR and GFP genes are both under pT7 promoter control. However, pT7 can only function under presence of associated regulatory protein; thus, for example, when we transform this construct into strain BL21 E. coli, which express associated regulator, both tetR and GFP will be expressed.In contrast, if we transform this construct into other strain, such as DH5alpha and S17-1, both tetR and GFP genes will not be expressed. Due to that tetR and GFP are under the regulation of the same promoter, we can quantitize the tetR expression by detection intensity of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1430