Difference between revisions of "Part:BBa K624056"

 
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pYMB essentials + RBS(Pmsp3) + minC
 
pYMB essentials + RBS(Pmsp3) + minC
  
pYMB is a shuttle vector for manipulating and transforming gene into AMB-1
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pYMB ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624004 BBa_K624004]) is a shuttle vector for manipulating and transforming gene into AMB-1.
RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter  
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of AMB-1  
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The ribosome binding site is from Pmsp3, the strongest promoter  
minC,the division inhibitor,its overexpression
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of AMB-1.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624012 BBa_K624012])
would lead to septation inhibition at all potential  
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division sites, a circumstance of filamentation.
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MinC ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624033 BBa_K624033]) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites.
The ribosome binding site is from Pmsp3.(BBa_K624012).
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<partinfo>BBa_K624056 parameters</partinfo>
 
<partinfo>BBa_K624056 parameters</partinfo>
 
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[[image:pymb_minc.jpg|500px|]]

Latest revision as of 19:46, 14 October 2011

pYMB essentials + RBS(Pmsp3) + minC

pYMB essentials + RBS(Pmsp3) + minC

pYMB (BBa_K624004) is a shuttle vector for manipulating and transforming gene into AMB-1.

The ribosome binding site is from Pmsp3, the strongest promoter of AMB-1.(BBa_K624012)

MinC (BBa_K624033) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal AgeI site found at 1398
  • 1000
    COMPATIBLE WITH RFC[1000]



Pymb minc.jpg