Difference between revisions of "Part:BBa K624056"

Latest revision as of 19:46, 14 October 2011

pYMB essentials + RBS(Pmsp3) + minC

pYMB (BBa_K624004) is a shuttle vector for manipulating and transforming gene into AMB-1.

The ribosome binding site is from Pmsp3, the strongest promoter of AMB-1.(BBa_K624012)

MinC (BBa_K624033) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 898
Illegal AgeI site found at 1398
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K624056 short</partinfo>
 pYMB essentials + RBS(Pmsp3) + minC
+pYMB ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624004 BBa_K624004]) is a shuttle vector for manipulating and transforming gene into AMB-1.
+The ribosome binding site is from Pmsp3, the strongest promoter
+of AMB-1.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624012 BBa_K624012])
+MinC ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624033 BBa_K624033]) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites.
 <!-- Add more about the biology of this part here
@@ Line 17: / Line 23: @@
 <partinfo>BBa_K624056 parameters</partinfo>
 <!-- -->
+[[image:pymb_minc.jpg|500px|]]