Difference between revisions of "Part:BBa K602013:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K602013=== | ===Applications of BBa_K602013=== | ||
| + | ==== 2011 Osaka ==== | ||
| + | This part was assayed for DNA damage detection ability as follows. <i>E. coli</i> transformed with this part was irradiated with UV light, and then incubated for 2 hours to provide sufficient time for lycopene production. Following that, the lycopene was extracted using acetone. The lycopene concentration was measured by absorbance at 474 nm and regarded as an approximate indicator of promoter activity. | ||
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| + | In general, a high background level of lycopene was measured even in the absence of any irradiation. This might be due to basal expression from the SOS promoter which is needed for the SOS genes' rapid response to damage. Therefore, promoter response to UV irradiation was defined as the fractional increase of lycopene production over non-irradiated controls, for each irradiated sample/UV energy dosage. | ||
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| + | [[Image:2011_osaka_promoter.png|600px]] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 19:27, 14 October 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K602013
2011 Osaka
This part was assayed for DNA damage detection ability as follows. E. coli transformed with this part was irradiated with UV light, and then incubated for 2 hours to provide sufficient time for lycopene production. Following that, the lycopene was extracted using acetone. The lycopene concentration was measured by absorbance at 474 nm and regarded as an approximate indicator of promoter activity.
In general, a high background level of lycopene was measured even in the absence of any irradiation. This might be due to basal expression from the SOS promoter which is needed for the SOS genes' rapid response to damage. Therefore, promoter response to UV irradiation was defined as the fractional increase of lycopene production over non-irradiated controls, for each irradiated sample/UV energy dosage.
User Reviews
UNIQ6795c7041e9c4437-partinfo-00000000-QINU UNIQ6795c7041e9c4437-partinfo-00000001-QINU