Difference between revisions of "Part:BBa K622005:Experience"
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We performed the function check of σ<sup>54</sup> promoter in the genome of ''E. coli''. | We performed the function check of σ<sup>54</sup> promoter in the genome of ''E. coli''. | ||
| − | By measuring the amount of mRNA of glnG, the promoter activities in the different glutamine concentration were measured (Fig.1). Figure 1 shows that the activities in 0.2 and 2.0 % glutamine are repressed to one-fifth of that in 0.0 % glutamine. | + | By measuring the amount of mRNA of glnG, the promoter activities in the different glutamine concentration were measured (Fig.1). Figure 1 shows that the activities in 0.2 and 2.0 % glutamine are repressed to less than one-fifth of that in 0.0 % glutamine. |
[[Image:Kyoto-sigma54-1.png|400px|thumb|center|Fig.1: σ<sup>54</sup> promoter activity.]] | [[Image:Kyoto-sigma54-1.png|400px|thumb|center|Fig.1: σ<sup>54</sup> promoter activity.]] | ||
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Characterization of σ54 promoter activity
We performed the function check of σ54 promoter in the genome of E. coli.
By measuring the amount of mRNA of glnG, the promoter activities in the different glutamine concentration were measured (Fig.1). Figure 1 shows that the activities in 0.2 and 2.0 % glutamine are repressed to less than one-fifth of that in 0.0 % glutamine.
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