Difference between revisions of "Part:BBa K622005:Experience"
(→Characterization of σ54 promoter activity) |
(→Characterization of σ54 promoter activity) |
||
| Line 6: | Line 6: | ||
We performed the function check of σ<sup>54</sup> promoter in the genome of ''E. coli''. | We performed the function check of σ<sup>54</sup> promoter in the genome of ''E. coli''. | ||
| − | By measuring the amount of mRNA of glnG, the promoter activities in the different glutamine concentration were measured (Fig.1). Figure 1 shows that the activities in 0.2 and 2.0 % glutamine are a | + | By measuring the amount of mRNA of glnG, the promoter activities in the different glutamine concentration were measured (Fig.1). Figure 1 shows that the activities in 0.2 and 2.0 % glutamine are repressed to a five of that in 0.0 % glutamine. |
[[Image:Kyoto-sigma54-1.png|400px|thumb|center|Fig.1: σ<sup>54</sup> promoter activity.]] | [[Image:Kyoto-sigma54-1.png|400px|thumb|center|Fig.1: σ<sup>54</sup> promoter activity.]] | ||
Revision as of 10:22, 14 October 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Characterization of σ54 promoter activity
We performed the function check of σ54 promoter in the genome of E. coli.
By measuring the amount of mRNA of glnG, the promoter activities in the different glutamine concentration were measured (Fig.1). Figure 1 shows that the activities in 0.2 and 2.0 % glutamine are repressed to a five of that in 0.0 % glutamine.
User Reviews
UNIQ3a446f5679ec0ead-partinfo-00000000-QINU UNIQ3a446f5679ec0ead-partinfo-00000001-QINU