Difference between revisions of "Part:BBa K649001"

 
 
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__NOTOC__
 
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<partinfo>BBa_K649001 short</partinfo>
 
<partinfo>BBa_K649001 short</partinfo>
  
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GFP reporter regulated by 3OC12-HSL and LasR.
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Fluorescence intensity of BBa_K649001 was increased by 3OC12-HSL induction. GFP expression after induction of 3OC12-HSL is 170 times as high as before.
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[[Image:BBa K649001 graph3.png|thumb|center|500px|Effect of 3OC12-HSL induction on fluorescence intensity<br>This work is done by Takuya Tsubaki.]]
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Generally, in the presence of 3OC12-HSL, LasR activates lasI promoter and the transcription level of downstream gene increases, but in the absence of 3OC12-HSL, LasR can't activate lasI promoter. 2011 iGEM Tokyo-Tech characterized BBa_K649000 by means of using BBa_K649001 composed of BBa_K649000 and BBa_J54103.
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We improved previous las promoters.<br>
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<ol>
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<li>
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[https://parts.igem.org/Part:BBa_J64010:Experience our assay of BBa_J64010]
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</li>
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<li>
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[https://parts.igem.org/Part:BBa_K091117 BBa_K091117]
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</li>
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<li>
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[https://parts.igem.org/Part:BBa_R0079 BBa_R0079]
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</li>
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<li>
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[https://parts.igem.org/Part:BBa_K195616 BBa_K195616]
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</li>
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<li>
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[https://parts.igem.org/Part:BBa_J69551 BBa_J69551]
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</li>
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</ol>
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For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#3. our work in Tokyo_Tech 2011 wiki].
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:47, 14 October 2011

GFP regulated by 3OC12-HSL and LasR

Fluorescence intensity of BBa_K649001 was increased by 3OC12-HSL induction. GFP expression after induction of 3OC12-HSL is 170 times as high as before.


Effect of 3OC12-HSL induction on fluorescence intensity
This work is done by Takuya Tsubaki.


Generally, in the presence of 3OC12-HSL, LasR activates lasI promoter and the transcription level of downstream gene increases, but in the absence of 3OC12-HSL, LasR can't activate lasI promoter. 2011 iGEM Tokyo-Tech characterized BBa_K649000 by means of using BBa_K649001 composed of BBa_K649000 and BBa_J54103.


We improved previous las promoters.

  1. our assay of BBa_J64010
  2. BBa_K091117
  3. BBa_R0079
  4. BBa_K195616
  5. BBa_J69551


For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#3. our work in Tokyo_Tech 2011 wiki].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 118
    Illegal BamHI site found at 106
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 805