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| | ===Applications of BBa_K676001=== | | ===Applications of BBa_K676001=== |
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| − | According to our result in the 1D chloroquine gel electrophoresis (at 2.5 μg/mL), the plasmid with the Mu phage GBS (on pSB1C3 backbone) migrated further compared to the control plasmid (pSB1C3 with RFP expression cassette - BBa_J04450). At the chloroquine concentration which we used, the more negatively supercoiled plasmid will migrate further while the more relaxed molecules will travel slower through the gel.
| + | [[Image:Characterising Part BBa_K676001.jpg]] |
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| − | '''The level of supercoiling achieved'''
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| − | The plasmids with Mu GBS travelled further compared to the control . This indicated that the Mu GBS enables a higher supercoiled topoisomers to be formed .There was also one distinct band which migrate the furthest from the wells and bands in the Mu GBS lane , this indicate that the introduction of Mu GBS can result in the formation of a highly supercoiled plasmids . Overall ,the level of negative supercoiling achieved with the Mu SGS was:
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| − | Calculations: By comparing the length of travel of the bands from the well in each samples
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| − | Control= 9.6cm
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| − | Mu GBS = 13.7cm
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| − | Difference: (4.2/9.6)(100) = 42.7%
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| − | '''Quality of supercoiling:'''
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| − | Besides achieving a higher level of supercoiling, the quality of the supercoiled plasmids also showed to be better. The uniformity or homogeneity of the supercoiled Mu GBS plasmids increased by close to 30%( if the single distinct band was not included) compared to the control.
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| − | Calculations: By comparing the range of topoisomers travel distances ( between the "slowest" and "fastest")
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| − | Control=4.9cm "smear"
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| − | Mu GBS =3.4cm "smear"
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| − | Difference: (1.5/4.9)(100%) = 30.6%
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| | ===User Reviews=== | | ===User Reviews=== |
Latest revision as of 02:25, 10 October 2011
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Applications of BBa_K676001
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