Difference between revisions of "Part:BBa K676001:Experience"

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===Applications of BBa_K676001===
 
===Applications of BBa_K676001===
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[[Image:UCL-Content-Chloroquine_Gel_-Comparing_GBS_and_control.jpg]]
 
  
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According to our result in the 1D chloroquine gel electrophoresis (at 2.5 μg/mL) , the plasmid with the Mu phage GBS( on pSB1C3 backbone) migrated further compared to the control plasmid (pSB1C3 with RFP expression cassette - BBa_J04450). At the chloroquine concentration which we used, the more negatively supercoiled plasmid will migrate further while the more relaxed molecules will travel slower through the gel.
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[[Image:Characterising Part BBa_K676001.jpg]]
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Besides achieving a higher level of supercoiling, the quality of the supercoiled plasmids also showed to be better. The uniformity or homogeneity of the supercoiled target plasmids increases by close to 40% compared to the control.
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===User Reviews===
 
===User Reviews===

Latest revision as of 02:25, 10 October 2011

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Please enter how you used this part and how it worked out.

Applications of BBa_K676001

Characterising Part BBa K676001.jpg

User Reviews

UNIQ404c818d5d22f3d1-partinfo-00000000-QINU UNIQ404c818d5d22f3d1-partinfo-00000001-QINU