Difference between revisions of "Part:BBa K676001"
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<partinfo>BBa_K676001 short</partinfo>
 
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The strong gyrase site clones from Mu bacteriophage.
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The strong gyrase site cloned from Mu bacteriophage genome.
  
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The Mu phage Gyrase Binding sites serve as a high affinity binding site for the DNA gyrase enzymes. The site will be cleaved preferentially by the GyrA and resulting in an introduction of linking number difference into the DNA by the topoisomerase.
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===Usage and Biology===
  
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Apparently some bacteriophages  exploited the GBS to allow them to replicate successfully in their host. For example, Mu – bacteriophage has a strong gyrase site or GBS in the central region of the 37.2kbp Mu phage genome.
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The Mu phage strong gyrase site (SGS) serves as a high affinity binding site for the DNA gyrase enzyme. This site is known to be cleaved preferentially by the Gyrase A subunits and therefore results in an increase in the linking number difference of the DNA molecule.
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From Pato(1990) it was found out that the wild type phage can carry out replication effectively compare to the mutant which lacks the GBS after the heat induction of the lysogens .  
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By introducing the Mu GBS into the target plasmids, we achieved a higher level of supercoiling as well as obtaining a more uniformly supercoiled products . Highly supercoiled plasmids are able to resist shear stress better and this property plays an important role in large scale plasmid DNA manufacturing as well as gene therapy delivery.
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Apparently, some bacteriophages are known to exploit the GBS to allow them to replicate successfully in their host. For example, Mu bacteriophage has a strong gyrase site or GBS in the central region of their 37.2 kbp genome. From work carried out by Pato et al. in 1990, it was found out that the wild type phage can carry out replication effectively compared a mutant which lacks the GBS after the heat induction of the lysogens.  
  
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By introducing the Mu GBS into the target plasmids, we managed to achiev a higher level of supercoiling as well as  better uniformity in the final supercoiled products. Highly supercoiled plasmids are able to resist shear stress better and this property plays an important role in large scale plasmid DNA manufacturing as well as for the delivery therapy of therapeutic genes.
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===Usage and Biology===
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[[Image:Characterising Part BBa_K676001.jpg]]
  
 
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[[Image:Characterising Part BBa_K676001.jpg]]
 

Latest revision as of 02:23, 10 October 2011

Gyrase Binding Site from Mu Bacteriophage

The strong gyrase site cloned from Mu bacteriophage genome.

Usage and Biology

The Mu phage strong gyrase site (SGS) serves as a high affinity binding site for the DNA gyrase enzyme. This site is known to be cleaved preferentially by the Gyrase A subunits and therefore results in an increase in the linking number difference of the DNA molecule.

Apparently, some bacteriophages are known to exploit the GBS to allow them to replicate successfully in their host. For example, Mu bacteriophage has a strong gyrase site or GBS in the central region of their 37.2 kbp genome. From work carried out by Pato et al. in 1990, it was found out that the wild type phage can carry out replication effectively compared a mutant which lacks the GBS after the heat induction of the lysogens.

By introducing the Mu GBS into the target plasmids, we managed to achiev a higher level of supercoiling as well as better uniformity in the final supercoiled products. Highly supercoiled plasmids are able to resist shear stress better and this property plays an important role in large scale plasmid DNA manufacturing as well as for the delivery therapy of therapeutic genes.

Characterising Part BBa K676001.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 191
  • 1000
    COMPATIBLE WITH RFC[1000]