Difference between revisions of "Part:BBa K676012:Design"

 
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===Design Notes===
 
===Design Notes===
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Design PCR
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Performed PCR to clone out the GBS from the pBR322 plasmid. Carried out 2 parts ligation between the cloned GBS and pSB1C3 plasmid backbone.
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===Source===
 
===Source===
  
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PCR and then 2 parts ligation.
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pBR322 Plasmid DNA
  
 
===References===
 
===References===
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Mark Oram, Alison J. Howells, Anthony Maxwell and Martin L . Pato (2003) A biochemical analysis of the interaction of DNA gyrase with the bacteriophage Mu, pSC101 and pBR322 Strong gyrase Sites; the role of DNA sequence in modulating gyrase supercoiling and biological activity; Molecular Microbiology 50 (1) 333-347

Latest revision as of 02:20, 10 October 2011

Gyrase Binding Site from pBR322


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 149
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Performed PCR to clone out the GBS from the pBR322 plasmid. Carried out 2 parts ligation between the cloned GBS and pSB1C3 plasmid backbone.

Source

pBR322 Plasmid DNA

References

Mark Oram, Alison J. Howells, Anthony Maxwell and Martin L . Pato (2003) A biochemical analysis of the interaction of DNA gyrase with the bacteriophage Mu, pSC101 and pBR322 Strong gyrase Sites; the role of DNA sequence in modulating gyrase supercoiling and biological activity; Molecular Microbiology 50 (1) 333-347