Difference between revisions of "Part:BBa K632002"

Revision as of 23:23, 9 October 2011

INP-Silicatein

The silicatein gene has been successfully fused to an existent ice nucleation protein (BBa_K265009). Gel electrophoresis and DNA sequencing both suggest the fusion product is the correct size, frame, and orientation. Silicatein functional assay was performed to verify the activity of silicatein, and the results indicate the Silicatein is enzymatically active.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1
Illegal XbaI site found at 7
Illegal SpeI site found at 2398
Illegal PstI site found at 1433
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1
Illegal NheI site found at 1798
Illegal SpeI site found at 2398
Illegal PstI site found at 1433
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1
Illegal BglII site found at 1623
Illegal BamHI site found at 337
Illegal XhoI site found at 1387
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1
Illegal XbaI site found at 7
Illegal SpeI site found at 2398
Illegal PstI site found at 1433
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1
Illegal XbaI site found at 7
Illegal SpeI site found at 2398
Illegal PstI site found at 1433
Illegal NgoMIV site found at 747
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K632002 short</partinfo>
-R
+The silicatein gene has been successfully fused to an existent ice nucleation protein (BBa_K265009). Gel electrophoresis and DNA sequencing both suggest the fusion product is the correct size, frame, and orientation. Silicatein functional assay was performed to verify the activity of silicatein, and the results indicate the Silicatein is enzymatically active.
 <!-- Add more about the biology of this part here