Difference between revisions of "Part:BBa K239009:Experience"

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This part works well and the fluorescence data from the fermentation was as expected. We compared the level of supercoiling and expression of the GFP in this device with a plasmid containing the device ligated to Mu phage GBS ([[Part:BBa_K676013]])
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Revision as of 23:16, 9 October 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K239009

This part was designed to test promoter spy, BBa_K239001. It can be used to detect misfolding of proteins in the periplasm or shear stress in bio-processing.

User Reviews


UNIQ65b45b0174a723b8-partinfo-00000001-QINU UNIQ65b45b0174a723b8-partinfo-00000002-QINU

The device responded to various periplasmic stresses such as Copper (4mM CuCl2), Indole (4mM) and Ethanol (5%). Possibly it can also be used to detect presence of heterogeneous unfolded proteins in the periplasmic space (e.g. target pharmaceuticals) or to detect high levels of shear stress during cultivation in a bioreactor.

Compare to the related DegP promoter (BBa_K239000), Spy promoter, which is dependent on sigma factor 70, can be induced up to 40 fold by over expression of CpxR. (Bury-Moné et al. 2009) Most importantly, spy promoter showed no respond to stationary phase.

For detailed information please visit [http://2009.igem.org/Team:UCL_London/From_the_lab/Results#Growth_Cruves_Experiments_II UCL_London iGEM Team Wiki Growth_Cruves_Experiments_II]

By iGEM11_UCL_London: