Difference between revisions of "Part:BBa K676013:Design"

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===Design Notes===
 
===Design Notes===
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AFter the PCR and ligation of Mu GBS into a pSB1C3 backbone, we carried out 3A Assembly of the Mu GBS with the SPY device in BBa_K239009 into a pSB1A3
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Performed 3A Assembly between the Mu phage GBS ([[Part:BBa_K676001]]) with the Spy promoter device ([[Part:BBa_K239009]]) constructed by iGEM09_UCL_London.
  
 
===Source===
 
===Source===

Revision as of 21:58, 9 October 2011

Mu/Spy Ligation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 191
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1203


Design Notes

Performed 3A Assembly between the Mu phage GBS (Part:BBa_K676001) with the Spy promoter device (Part:BBa_K239009) constructed by iGEM09_UCL_London.

Source

2009 UCL SPY device

References