Difference between revisions of "Part:BBa K624047"

Revision as of 12:51, 9 October 2011

RBS(pmsp3) + LLO + RBS(pmsp3) + Inv + RBS(pmsp3) + ECFP

RBS(pmsp3) + LLO + RBS(pmsp3) + Inv + ECFP

Listeriolysin O (LLO) is a hemolysin produced by the bacterium Listeria monocytogenes, a pathogen that is responsible for listeriosis. LLO is activated within phagosomes of cells that have phagocytosed L. monocytogenes cells, and lyses the membrane of phagosome. The bacteria is then able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. This would result in the protection from extracellular immune system.

Invasin is an outer membrane protein that allows bacteria to penetrate mammalian cells. It is from Yersinia enterocolitica. Invasin binds to multiple Beta 1 chain integrin receptors on mammalian cells with high affinity, which induces endocytosis and internalization.

All of the ribosome binding sites are from Pmsp3, the strongest promoter of AMB-1. (BBa_K624012)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1417
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2423
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 3040
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K624047 short</partinfo>
 RBS(pmsp3) + LLO + RBS(pmsp3) + Inv + ECFP
+Listeriolysin O (LLO) is a hemolysin produced by the bacterium ''Listeria monocytogenes'', a pathogen that is responsible for listeriosis. LLO is activated within phagosomes of cells that have phagocytosed ''L. monocytogenes'' cells, and lyses the membrane of phagosome. The bacteria is then able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. This would result in the protection from extracellular immune system.
+Invasin is an outer membrane protein that allows bacteria to penetrate mammalian cells. It is from ''Yersinia enterocolitica''. Invasin binds to multiple Beta 1 chain integrin receptors on mammalian cells with high affinity, which induces endocytosis and internalization.
+All of the ribosome binding sites are from Pmsp3, the strongest promoter of AMB-1. ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624012 BBa_K624012])
 <!-- Add more about the biology of this part here