Difference between revisions of "Part:BBa K624041"

Latest revision as of 12:40, 9 October 2011

pT7-tetO+RBS+minC+mcherry

This construct is composed of pT7-tetO, ribosome binding site, minC cell division inhibitor, and mcherry reporter. pT7+RBS+tetR+RBS+GFP+Ter (BBa_K624002) regulates this construct by producing tet repressor in the presence of tetracycline.

MinC (BBa_K624033) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 259
1000
COMPATIBLE WITH RFC[1000]

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 This construct is composed of pT7-tetO, ribosome binding site, minC cell division inhibitor, and mcherry reporter.
-pT7+RBS+tetR+RBS+GFP+Ter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624002 BBa_K624002]) regulates this construct by produce tet repressor in the presence of tetracycline.
+pT7+RBS+tetR+RBS+GFP+Ter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624002 BBa_K624002]) regulates this construct by producing tet repressor in the presence of tetracycline.
 MinC ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624033 BBa_K624033]) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites.