Difference between revisions of "Part:BBa K624018"

Latest revision as of 12:38, 9 October 2011

pYMB essentials+[YN-13-R]+[YC-CHAMP]

pYMB indicates pUC19 with ori+Pmsp1+rep. In this construct, YN-13-R and YC-CHAMP are ligated in the downstream of ori+Pmsp1+rep, aimed at serving as optogenetic wireless neurostimulator with OFF state (no BRET between EYFP and rLuc) as default mode. BiFC between YN and YC is anticipated in the default state as well. But according to the opposite location of YC on CHAMP, this part is intended for a negative control, also testing the orientation of CHAMP on the effect of BiFC-based BRET between EYFP and rLuc.

Fig. 3: The main design construct. Ori is the origin of replication of AMB-1. Pmsp1 is the promoter for AMB-1.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 898
Illegal NgoMIV site found at 1824
Illegal NgoMIV site found at 1908
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2577

@@ Line 3: / Line 3: @@
 pYMB indicates pUC19 with ori+Pmsp1+rep. In this construct, YN-13-R and YC-CHAMP are ligated in the downstream of ori+Pmsp1+rep, aimed at serving as optogenetic wireless neurostimulator with OFF state (no BRET between EYFP and rLuc) as default mode. BiFC between YN and YC is anticipated in the default state as well. But according to the opposite location of YC on CHAMP, this part is intended for a negative control, also testing the orientation of CHAMP on the effect of BiFC-based BRET between EYFP and rLuc.
+[[Image: c1_NYMU.png|frame|none|Fig. 3: The main design construct. Ori is the origin of replication of AMB-1. Pmsp1 is the promoter for AMB-1.]]
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