Difference between revisions of "Part:BBa K624055"

Latest revision as of 12:27, 9 October 2011

pYMB essentials + RBS(Pmsp3) + tetR + RBS(Pmsp3) + GFP

Pmsp1 + RBS(Pmsp3) + tetR + RBS(Pmsp3) + GFP

The Pmsp1 is the second strongest promoter on AMB-1.

RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter of AMB-1.(BBa_K624012)

TetR serves as a switch, which can turn on the downstream gene when tetracycline is present; and vice versa.

GFP serves as a reporter to show if the construct work or not.

Sequence and Features

Assembly Compatibility:

@@ Line 4: / Line 4: @@
 Pmsp1 + RBS(Pmsp3) + tetR + RBS(Pmsp3) + GFP
-The Pmsp1 is the second strongest promoter
+The Pmsp1 is the second strongest promoter on AMB-1.
-on AMB-1.
-RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter
+RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter of AMB-1.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624012 BBa_K624012])
-of AMB-1
-tetR serves as a switch,which can turn on the downstream gene
+TetR serves as a switch, which can turn on the downstream gene
-when  Tetracycline is present ;and vice versa.
+when tetracycline is present; and vice versa.
 GFP serves as a reporter to show if the construct work or not.
-All of the ribosome binding sites are RBS from Pmsp3.(BBa_K624012).
 <!-- Add more about the biology of this part here