Difference between revisions of "Part:BBa K624055"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K624055 short</partinfo> | <partinfo>BBa_K624055 short</partinfo> | ||
| − | Pmsp1 + RBS + tetR + RBS + GFP | + | Pmsp1 + RBS(Pmsp3) + tetR + RBS(Pmsp3) + GFP |
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| + | The Pmsp1 is the second strongest promoter on AMB-1. | ||
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| + | RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter of AMB-1.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624012 BBa_K624012]) | ||
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| + | TetR serves as a switch, which can turn on the downstream gene | ||
| + | when tetracycline is present; and vice versa. | ||
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| + | GFP serves as a reporter to show if the construct work or not. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 12:27, 9 October 2011
pYMB essentials + RBS(Pmsp3) + tetR + RBS(Pmsp3) + GFP
Pmsp1 + RBS(Pmsp3) + tetR + RBS(Pmsp3) + GFP
The Pmsp1 is the second strongest promoter on AMB-1.
RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter of AMB-1.(BBa_K624012)
TetR serves as a switch, which can turn on the downstream gene when tetracycline is present; and vice versa.
GFP serves as a reporter to show if the construct work or not.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 898
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2561