Difference between revisions of "Part:BBa K624056"
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The ribosome binding site is from Pmsp3, the strongest promoter | The ribosome binding site is from Pmsp3, the strongest promoter | ||
| − | of AMB-1 | + | of AMB-1.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624012 BBa_K624012]) |
MinC ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624033 BBa_K624033]) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites. | MinC ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624033 BBa_K624033]) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites. | ||
Revision as of 12:23, 9 October 2011
pYMB essentials + RBS(Pmsp3) + minC
pYMB essentials + RBS(Pmsp3) + minC
pYMB (BBa_K624004) is a shuttle vector for manipulating and transforming gene into AMB-1.
The ribosome binding site is from Pmsp3, the strongest promoter of AMB-1.(BBa_K624012)
MinC (BBa_K624033) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 898
Illegal AgeI site found at 1398 - 1000COMPATIBLE WITH RFC[1000]