Difference between revisions of "Part:BBa K624058"

Revision as of 12:18, 9 October 2011

pYMB essentials + RBS(Pmsp3) + Inv

pYMB (BBa_K624004)is a shuttle vector for manipulating and transforming gene into AMB-1.

The ribosome binding site is from Pmsp3, the strongest promoter of AMB-1.(BBa_K624012).

Invasin is an outer membrane protein that allows bacteria to penetrate mammalian cells. It is from Yersinia enterocolitica. Invasin binds to multiple Beta 1 chain integrin receptors on mammalian cells with high affinity, which induces endocytosis and internalization.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1938
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 898
Illegal NgoMIV site found at 2555
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 pYMB essentials + RBS(Pmsp3) + Inv
-pYMB  is a shuttle vector for manipulating and transforming gene into AMB-1.
+pYMB ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624004 BBa_K624004])is a shuttle vector for manipulating and transforming gene into AMB-1.
-RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter
-of AMB-1.
-Inv(Invasin), is an outer membrane protein found on the
-surface of Y. enterocolitica that is responsible for
-its binding property.
-The ribosome binding site is from Pmsp3.(BBa_K624012)
-The ribosome binding site is from Pmsp3.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624012 BBa_K624012]).
+The ribosome binding site is from Pmsp3, the strongest promoter
+of AMB-1.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624012 BBa_K624012]).
 Invasin is an outer membrane protein that allows bacteria to penetrate mammalian cells. It is from ''Yersinia enterocolitica''. Invasin binds to multiple Beta 1 chain integrin receptors on mammalian cells with high affinity, which induces endocytosis and internalization.