Difference between revisions of "Part:BBa K624033"
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We have cloned the ''minC'' gene from E. coli K-12 strain MG1655 by performing PCR, with a single nucleotide synonymous mutation to eliminate the PstI site. | We have cloned the ''minC'' gene from E. coli K-12 strain MG1655 by performing PCR, with a single nucleotide synonymous mutation to eliminate the PstI site. | ||
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| + | Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites. | ||
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Revision as of 11:57, 9 October 2011
minC cell division inhibitor (revised)
This part, minC, is known as a cell division inhibitor. Its first record on iGEM was made by 2006 Chiba iGEM team(BBa_J29040), but there is not an available part. 2010 Warsaw iGEM team submitted another minC (BBa_K299806) with different sequence. However, there was a PstI site in the middle of the sequence.
We have cloned the minC gene from E. coli K-12 strain MG1655 by performing PCR, with a single nucleotide synonymous mutation to eliminate the PstI site.
Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 211
- 1000COMPATIBLE WITH RFC[1000]