Difference between revisions of "Part:BBa K624056"

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pYMB essentials + RBS(Pmsp3) + minC
 
pYMB essentials + RBS(Pmsp3) + minC
  
The ribosome binding site is from Pmsp3.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624012 BBa_K624012]).
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pYMB is a shuttle vector for manipulating and transforming gene into AMB-1
 +
RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter
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of AMB-1
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minC,the division inhibitor,its overexpression
 +
would lead to septation inhibition at all potential
 +
division sites, a circumstance of filamentation.
 +
The ribosome binding site is from Pmsp3.(BBa_K624012).
  
 
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Revision as of 11:20, 9 October 2011

pYMB essentials + RBS(Pmsp3) + minC

pYMB essentials + RBS(Pmsp3) + minC

pYMB is a shuttle vector for manipulating and transforming gene into AMB-1 RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter of AMB-1 minC,the division inhibitor,its overexpression would lead to septation inhibition at all potential division sites, a circumstance of filamentation. The ribosome binding site is from Pmsp3.(BBa_K624012).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal AgeI site found at 1398
  • 1000
    COMPATIBLE WITH RFC[1000]