Difference between revisions of "Part:BBa K624044"
| Line 3: | Line 3: | ||
ori + Pmsp1 + rep + RBS + LLO + RBS + Invasin + ECFP | ori + Pmsp1 + rep + RBS + LLO + RBS + Invasin + ECFP | ||
| + | |||
The ori serve as the Origin of Replication,and | The ori serve as the Origin of Replication,and | ||
the Pmsp1 is the second strongest promoter | the Pmsp1 is the second strongest promoter | ||
Revision as of 11:17, 9 October 2011
pYMB essentials + RBS + LLO + RBS + Invasin + RBS + ECFP
ori + Pmsp1 + rep + RBS + LLO + RBS + Invasin + ECFP
The ori serve as the Origin of Replication,and the Pmsp1 is the second strongest promoter on AMB-1. LLO (Listeriolysin O) can make the bacteria be able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. which help protect the AMB-1 from extracellular immune system. ECFP serves as a reporter to show if the construct work or not.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2567
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3573
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 898
Illegal NgoMIV site found at 4190 - 1000COMPATIBLE WITH RFC[1000]