Difference between revisions of "Part:BBa K539151:Design"

(New page: __NOTOC__ <partinfo>BBa_K539151 short</partinfo> <partinfo>BBa_K539151 SequenceAndFeatures</partinfo> ===Design Notes=== In our experiment, the intensity of RNA thermometer are °C, 42...)
 
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===Design Notes===
 
===Design Notes===
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In our experiment, the intensity of RNA thermometer are °C, 42°C, 32°C, 27°C.
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In our experiment, the order of intensity of RNA thermometer are 37°C, 42°C, 32°C, 27°C.
 
With the help of RNA thermometer, we can control the color of pigment by different temperatures. When the temperature is lower than 37°C, the pigment is red because the translation of crtEBI. When the temperature is higher than 37°C, the pigment is orange because of the translation of crtY that convert lycopene to orange beta-carotene.
 
With the help of RNA thermometer, we can control the color of pigment by different temperatures. When the temperature is lower than 37°C, the pigment is red because the translation of crtEBI. When the temperature is higher than 37°C, the pigment is orange because of the translation of crtY that convert lycopene to orange beta-carotene.
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===Source===
 
===Source===

Latest revision as of 10:59, 8 October 2011

promoter+crtEBI+RNA thermometer(FourU)+crtY+terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2017
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1553
    Illegal NgoMIV site found at 1683
    Illegal NgoMIV site found at 4654
    Illegal AgeI site found at 768
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our experiment, the order of intensity of RNA thermometer are 37°C, 42°C, 32°C, 27°C. With the help of RNA thermometer, we can control the color of pigment by different temperatures. When the temperature is lower than 37°C, the pigment is red because the translation of crtEBI. When the temperature is higher than 37°C, the pigment is orange because of the translation of crtY that convert lycopene to orange beta-carotene.

Source

Constitutive promoter BBa_J23103 + CrtEBI(BBa_K274100) comes from team Peking 2010. Other parts come from parts-registry.

References