Difference between revisions of "Part:BBa K539714:Design"

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===Design Notes===
 
===Design Notes===
 
Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding sequence, those RE site should not exist in the coding region.There is one EcoR1 site in kivd. The RE site in the part has been modified by point mutation.
 
Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding sequence, those RE site should not exist in the coding region.There is one EcoR1 site in kivd. The RE site in the part has been modified by point mutation.
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<br>https://static.igem.org/mediawiki/parts/6/62/Kivd.png
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===Source===
 
===Source===
  
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It is cloned form Lactococcus lactis subsp, lactis KF147 by Zeng lab in NCTU.
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It is cloned form Lactococcus lactis subsp, lactis KF147 by Zeng lab, NCTU-Formosa.
  
 
===References===
 
===References===

Latest revision as of 09:47, 8 October 2011

kivd (alpha-ketoisovalerate decarboxylase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 578
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding sequence, those RE site should not exist in the coding region.There is one EcoR1 site in kivd. The RE site in the part has been modified by point mutation.
Kivd.png

Source

It is cloned form Lactococcus lactis subsp, lactis KF147 by Zeng lab, NCTU-Formosa.

References