Difference between revisions of "Part:BBa K539713:Design"

 
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<partinfo>BBa_K539713 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
 
Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding sequence, those RE site should not exist in coding sequence. There are two Pst1 site, one Xba1 site and one EcoR1 site in original sequence of AlsS. However, those RE site in AlsS has been modified by point mutation.
 
Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding sequence, those RE site should not exist in coding sequence. There are two Pst1 site, one Xba1 site and one EcoR1 site in original sequence of AlsS. However, those RE site in AlsS has been modified by point mutation.
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<br>https://static.igem.org/mediawiki/parts/f/fa/Alss-2.png
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===Source===
 
===Source===
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The gene is cloned by Zeng lab, NCTU-Formosa.
 
It comes from Bacillus subtilis.
 
It comes from Bacillus subtilis.
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===References===
 
===References===

Latest revision as of 09:45, 8 October 2011

AlsS (alpha-acetolactate synthase protein)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 308
    Illegal BsaI.rc site found at 902


Design Notes

Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding sequence, those RE site should not exist in coding sequence. There are two Pst1 site, one Xba1 site and one EcoR1 site in original sequence of AlsS. However, those RE site in AlsS has been modified by point mutation.
Alss-2.png

Source

The gene is cloned by Zeng lab, NCTU-Formosa. It comes from Bacillus subtilis.

References