Difference between revisions of "Part:BBa K539714"
 
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<partinfo>BBa_K539714 short</partinfo>
 
<partinfo>BBa_K539714 short</partinfo>
  
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This is the coding sequence of kivd from Lactococcus lactis subsp. It encodes alpha-ketoisovalerate decarboxylase which is responsible for the decarboxylation into aldehydes of &#945;-keto acids derived from amino acid transamination. This is part of the isobutanol biosynthesis pathway.  
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This is the coding sequence of ''kivd '' from Lactococcus lactis subsp. It encodes alpha-ketoisovalerate decarboxylase which is responsible for the decarboxylation into aldehydes of &#945;-keto acids derived from amino acid transamination. This gene plays the vital part of the isobutanol biosynthesis pathway.  
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Following is the isobutanol biosythesis pathway.
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<br>https://static.igem.org/mediawiki/parts/b/bc/Pathway%28small%29.jpg
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<br> Our team has mutate the function of RE site within the original '' kivd ''.
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<br>https://static.igem.org/mediawiki/parts/6/62/Kivd.png
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 09:19, 8 October 2011

kivd (alpha-ketoisovalerate decarboxylase)

This is the coding sequence of kivd from Lactococcus lactis subsp. It encodes alpha-ketoisovalerate decarboxylase which is responsible for the decarboxylation into aldehydes of α-keto acids derived from amino acid transamination. This gene plays the vital part of the isobutanol biosynthesis pathway. Following is the isobutanol biosythesis pathway.
Pathway%28small%29.jpg
Our team has mutate the function of RE site within the original kivd .
Kivd.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 578
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]