Difference between revisions of "Part:BBa K539714"
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<partinfo>BBa_K539714 short</partinfo> | <partinfo>BBa_K539714 short</partinfo> | ||
− | This is the coding sequence of kivd from Lactococcus lactis subsp. It encodes alpha-ketoisovalerate decarboxylase which is responsible for the decarboxylation into aldehydes of α-keto acids derived from amino acid transamination. This gene plays the vital part of the isobutanol biosynthesis pathway. | + | This is the coding sequence of "kivd" from Lactococcus lactis subsp. It encodes alpha-ketoisovalerate decarboxylase which is responsible for the decarboxylation into aldehydes of α-keto acids derived from amino acid transamination. This gene plays the vital part of the isobutanol biosynthesis pathway. |
Following is the isobutanol biosythesis pathway. | Following is the isobutanol biosythesis pathway. | ||
− | + | https://static.igem.org/mediawiki/parts/e/ec/Pathway.png | |
<br> Our team has mutate the function of RE site within the original "kivd". | <br> Our team has mutate the function of RE site within the original "kivd". | ||
− | <br>https://static.igem.org/mediawiki/parts/ | + | <br>https://static.igem.org/mediawiki/parts/6/62/Kivd.png |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 09:13, 8 October 2011
kivd (alpha-ketoisovalerate decarboxylase)
This is the coding sequence of "kivd" from Lactococcus lactis subsp. It encodes alpha-ketoisovalerate decarboxylase which is responsible for the decarboxylation into aldehydes of α-keto acids derived from amino acid transamination. This gene plays the vital part of the isobutanol biosynthesis pathway.
Following is the isobutanol biosythesis pathway.
Our team has mutate the function of RE site within the original "kivd".
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 578
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]