Difference between revisions of "Part:BBa K539119"
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We found a point mutation of [BBa_K118008] [https://parts.igem.org/Part:BBa_K118008](iGEM08_Edinburgh) at SpeI site that mutate into ACCTAGT. However, We corrected it successfully(ACCTAGT->ACTAGT), and submited to igem. | We found a point mutation of [BBa_K118008] [https://parts.igem.org/Part:BBa_K118008](iGEM08_Edinburgh) at SpeI site that mutate into ACCTAGT. However, We corrected it successfully(ACCTAGT->ACTAGT), and submited to igem. | ||
| − | + | <br><br>The following is the gel electrophoresis result of crtY [[Part:BBa_K118008]]. The sample included are uncut plasmid, cut by EcoR1, Xba1, Spe1 and Pst1 respctivly. | |
| + | <br><br>https://static.igem.org/mediawiki/parts/8/83/Crty_mutate-1.png | ||
| + | <br> We can see that the location of plasmid cut by Spe1 is the same as uncut plasmid. We assumed that Spe1 site has mutated. | ||
| + | <br>After the Point Mutation, we correct the Spe1 site and the gel electrophoresis result is shown below. | ||
| + | <br>https://static.igem.org/mediawiki/parts/c/cb/Crty_mutate-2.2.png | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 07:22, 8 October 2011
crtY (lycopene cyclase)
This is the coding sequence of crtY from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes lycopene B-cyclase, part of the carotenoid biosynthesis pathway, which converts lycopene to B-carotene (Misawa, N., Nakagawa, N., Kobayashi, K., Yamano, S., Nakamura, K., and Harashima, K. 1990. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. Journal of Bacteriology 172, 6704-612).
We found a point mutation of [BBa_K118008] [1](iGEM08_Edinburgh) at SpeI site that mutate into ACCTAGT. However, We corrected it successfully(ACCTAGT->ACTAGT), and submited to igem.
The following is the gel electrophoresis result of crtY Part:BBa_K118008. The sample included are uncut plasmid, cut by EcoR1, Xba1, Spe1 and Pst1 respctivly.
We can see that the location of plasmid cut by Spe1 is the same as uncut plasmid. We assumed that Spe1 site has mutated.
After the Point Mutation, we correct the Spe1 site and the gel electrophoresis result is shown below.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]