Difference between revisions of "Part:BBa K572100"
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+ | _NOTOC__ | ||
+ | <partinfo>BBa_K572100 short</partinfo> | ||
+ | |||
+ | The cloning vector was designed using pSB1C3 backbone. The antibiotic resistance gene is replaced by the proteorhodopsin gene so that the gene gives a metabolic advantage to the transformants on growing in minimal media. | ||
+ | |||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K572100 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K572100 parameters</partinfo> | ||
+ | <!-- --> | ||
<html> | <html> | ||
<img src="https://static.igem.org/mediawiki/parts/3/3e/PSB1PR1.GIF" WIDTH="525PX" HEIGHT="525PX"> | <img src="https://static.igem.org/mediawiki/parts/3/3e/PSB1PR1.GIF" WIDTH="525PX" HEIGHT="525PX"> |
Revision as of 04:08, 6 October 2011
_NOTOC__ Light based screening plasmid backbone - pSB1Pc
The cloning vector was designed using pSB1C3 backbone. The antibiotic resistance gene is replaced by the proteorhodopsin gene so that the gene gives a metabolic advantage to the transformants on growing in minimal media.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2139
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2145 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2139
Illegal XhoI site found at 1033
Illegal XhoI site found at 2015 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 2139
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2139
Plasmid lacks a suffix.
Illegal XbaI site found at 2154
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
The cloning vector was designed using pSB1C3 backbone. The antibiotic resistance gene is replaced by the proteorhodopsin gene so that the gene gives a metabolic advantage to the transformants on growing in minimal media.