Difference between revisions of "Part:BBa K572004"

 
 
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<partinfo>BBa_K572004 short</partinfo>
 
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The part is used as a control in our experiments with proteorhodopsin. A heat stress is introduced in cells which don't express proteorhodopsin to produce the recombinant protein, RFP and the productivity is compared to a system with proteorhodopsin.
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The part was designed to use it as a control in our experiments with proteorhodopsin. RFP production is estimated using luciferase activity.
  
 
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<partinfo>BBa_K572004 parameters</partinfo>
 
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The biobrick in pSB1A2 was transformed into DH5alpha cells. This primary culture was used as an inoculum for the secondary culture. After the OD reached 0.6, the cells were resuspended in M9 Media with different glucose concentrations - 0, 0.02, 0.1, 0.5, LB and LB+0.2.
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The biobrick contains a eukaryotic ribosome site. This experiment serves as a characterization of the ribosome binding site. Luciferase Assay showed basal level expression of the enzyme even after 6 hours after introducing cells into M9 media. The eukaryotic ribosome binding site didn't result in expression of the protein following it in E.coli.

Latest revision as of 03:10, 6 October 2011

PcstA_Luciferase Generator

The part was designed to use it as a control in our experiments with proteorhodopsin. RFP production is estimated using luciferase activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 684



The biobrick in pSB1A2 was transformed into DH5alpha cells. This primary culture was used as an inoculum for the secondary culture. After the OD reached 0.6, the cells were resuspended in M9 Media with different glucose concentrations - 0, 0.02, 0.1, 0.5, LB and LB+0.2.

The biobrick contains a eukaryotic ribosome site. This experiment serves as a characterization of the ribosome binding site. Luciferase Assay showed basal level expression of the enzyme even after 6 hours after introducing cells into M9 media. The eukaryotic ribosome binding site didn't result in expression of the protein following it in E.coli.