Difference between revisions of "Part:BBa K567017"

Latest revision as of 02:52, 6 October 2011

PT7-RFP-6AGG

RFP with 6 AGG-codon insertions after ATG under the control of T7 promoter and lac operator. The protein can be expressed successfully when co-transformed with PT7-TDRS (BBa_K567011) and tRNA(Asp)-AGG (BBa_K567012). Modified RFP exhibits lower red fluorescence brightness. This part is used to testify the function of PT7-TDRS (BBa_K567011).

Related Biobrick:

lacI-Ptrc-tRNA^Arg (BBa_K567001)

PT7-TDRS (BBa_K567011)

tRNA^Asp-AGG (BBa_K567012)

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 983
Illegal XbaI site found at 48
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 983
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 983
Illegal XhoI site found at 845
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 983
Illegal XbaI site found at 48
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 983
Illegal XbaI site found at 48
Illegal AgeI site found at 721
Illegal AgeI site found at 833
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K567017 short</partinfo>
 RFP with 6 AGG-codon insertions after ATG under the control of T7 promoter and lac operator. The protein can be expressed successfully when co-transformed with PT7-TDRS (BBa_K567011) and tRNA(Asp)-AGG (BBa_K567012). Modified RFP exhibits lower red fluorescence brightness. This part is used to testify the function of PT7-TDRS (BBa_K567011).
+Related Biobrick:
+''lacI''-Ptrc-tRNA<sup>Arg</sup>  ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K567001 BBa_K567001])
+PT7-TDRS  ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K567011 BBa_K567011])
+tRNA<sup>Asp</sup>-AGG  ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K567012 BBa_K567012])
 <!-- Add more about the biology of this part here