Difference between revisions of "Part:BBa K567012"
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tRNA(Asp) with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of ''aspV'' promoter. This biobrick is constructed first by cloning the tRNA(Asp) from ''aspV'' in E.coli, then the anticodon region is site-directed mutated.  
 
tRNA(Asp) with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of ''aspV'' promoter. This biobrick is constructed first by cloning the tRNA(Asp) from ''aspV'' in E.coli, then the anticodon region is site-directed mutated.  
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Get more information from Biobrick
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[https://parts.igem.org/wiki/index.php?title=Part:BBa_K567011 BBa_K567011]
  
 
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Revision as of 02:26, 6 October 2011

tRNA(Asp)-AGG

tRNA(Asp) with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of aspV promoter. This biobrick is constructed first by cloning the tRNA(Asp) from aspV in E.coli, then the anticodon region is site-directed mutated.

Get more information from Biobrick BBa_K567011

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 281
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 281
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 281
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 281
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 281
  • 1000
    COMPATIBLE WITH RFC[1000]