Difference between revisions of "Part:BBa K629005"

Revision as of 01:10, 6 October 2011

trkD, a functional Kup (formerly TrkD) system took up Cs+ with a moderate rate and affinity

Background

Escherichia coli cells which contain a functional Kup (formerly TrkD) system took up Cs' with a moderate rate and affinity. Kup is a separate K+ uptake system with relatively little discrimination in the transport of the cations K+, Rb+, and Cs'. Regardless of the presence or absence of Kup, K+-replete cells took up Cs'primarily by a very low affinity mode, proportional to the ratio of the Cs' and K+ concentrations in the medium. In our project, trkD is used to collect Cs-137 when recN is activated by radiation and start the transcription of trkD,in order to absorb this kind of radioactive source like Cs-137.

Expression in E. Coli

Before we started our functional test of TrkD, we have utilized western blot to determine whether there are enough soluble proteins in E. Coli. With 6×His tag on pET-32a, the result shows that TrkD has more expression with induction of IPTG.

Note: Because the promoter is Plac here, the expression has a background, which means control also expresses some TrkD without induction of IPTG.

Detailed Paramers in Result Report

Sequence and Features