Difference between revisions of "Part:BBa K567012"
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<partinfo>BBa_K567012 short</partinfo> | <partinfo>BBa_K567012 short</partinfo> | ||
− | tRNA(Asp) with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of | + | tRNA(Asp) with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of ''aspV'' promoter. This biobrick is constructed first by cloning the tRNA(Asp) from AspV in E.coli, then the anticodon region is site-directed mutated. |
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Revision as of 00:55, 6 October 2011
tRNA(Asp)-AGG
tRNA(Asp) with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of aspV promoter. This biobrick is constructed first by cloning the tRNA(Asp) from AspV in E.coli, then the anticodon region is site-directed mutated.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 281
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 281
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 281
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 281
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 281
- 1000COMPATIBLE WITH RFC[1000]