Difference between revisions of "Part:BBa K581003"
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<p><b>This BioBrick has been sequence verified.</b>
 
<p><b>This BioBrick has been sequence verified.</b>
  
 
<h2>Background</h2>
 
<h2>Background</h2>
  
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<p>SgrS is an Hfq-binding small antisense RNA that is induced upon phosphosugar stress (Vanderpool, 2007). It forms a ribonucleoprotein complex with RNase E through Hfq to mediate silencing of the target ptsG mRNA encoding the major glucose transporter (Geissmann and Touati, 2004). A 31-nt-long stretch in the 3’ region of SgrS is partially complementary to the translation initiation region of ptsG mRNA, and a 6 nt region overlapping the Shine-Dalgarno sequence of the target mRNA turns out to be crucial for SgrS’ function, shown as Fig 1 (Kawamoto et al., 2006; Maki et al., 2010).</p>
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<p>SgrS is an Hfq-binding small antisense RNA that is induced upon phosphosugar stress (Vanderpool, 2007). It forms a ribonucleoprotein complex with RNase E through Hfq to mediate silencing of the target ptsG mRNA encoding the major glucose transporter (Geissmann and Touati, 2004). A 31-nt-long stretch in the 3’ region of SgrS is partially complementary to the translation initiation region of ptsG mRNA, and a 6 nt region overlapping the Shine-Dalgarno sequence of the target mRNA turns out to be crucial for SgrS’ function, shown as Fig 2 (Kawamoto et al., 2006; Maki et al., 2010).</p>
  
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[[File:M_Mechanism.png‎|680px]]
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<img src="http://fmn.rrfmn.com/fmn058/20111006/0600/original_Y0mV_61420000b3931211.jpg" width="240px" align="right"/></a></h3>
  
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[[File:M_P1_S1.png|680px]]
 
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[[File:M_P2_S2.png‎|680px]]
 
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''Fig. 2 Sequence alignment of wild type ptsG/SgrS pair and its mutant complementary pairs.''
 
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''(A) The partial complementary region of ptsG (wt) mRNA and its corresponding sRNA SgrS.''
 
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''(B) The complementary pair of ptsG1 mRNA and corresponding SgrS1.''
 
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''(C) Another complementary pair site-mutant version of ptsG2 mRNA and corresponding SgrS2.''
 
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<p> miao </p>
 
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<h2>Experimental Data</h2>
 
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<h3>Methods<a href="http://2011.igem.org/Team:Peking_S/lab/protocol"><img src="__NOTOC__
 
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<partinfo>BBa_K581003 short</partinfo>
 
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This is the conjugate part of ptsG2-gfp.
 
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K581003 SequenceAndFeatures</partinfo>
 
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===Functional Parameters===
 
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<partinfo>BBa_K581003 parameters</partinfo>
 
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<html>
 
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<p><b>This BioBrick has been sequence verified.</b>
 
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<h2>Background</h2>
 
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<p>SgrS is an Hfq-binding small antisense RNA that is induced upon phosphosugar stress (Vanderpool, 2007). It forms a ribonucleoprotein complex with RNase E through Hfq to mediate silencing of the target ptsG mRNA encoding the major glucose transporter (Geissmann and Touati, 2004). A 31-nt-long stretch in the 3’ region of SgrS is partially complementary to the translation initiation region of ptsG mRNA, and a 6 nt region overlapping the Shine-Dalgarno sequence of the target mRNA turns out to be crucial for SgrS’ function, shown as Fig 2 (Kawamoto et al., 2006; Maki et al., 2010).</p>
 
  
 
<p> miao </p>
 
<p> miao </p>

Revision as of 22:18, 5 October 2011

SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)

This is the conjugate part of ptsG2-gfp.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


This BioBrick has been sequence verified.

Background

SgrS is an Hfq-binding small antisense RNA that is induced upon phosphosugar stress (Vanderpool, 2007). It forms a ribonucleoprotein complex with RNase E through Hfq to mediate silencing of the target ptsG mRNA encoding the major glucose transporter (Geissmann and Touati, 2004). A 31-nt-long stretch in the 3’ region of SgrS is partially complementary to the translation initiation region of ptsG mRNA, and a 6 nt region overlapping the Shine-Dalgarno sequence of the target mRNA turns out to be crucial for SgrS’ function, shown as Fig 2 (Kawamoto et al., 2006; Maki et al., 2010).

miao

Experimental Data

Methods

References

[1] Geissmann, T.A., and Touati, D. (2004). Hfq, a new chaperoning role: binding to messenger RNA determines access for small RNA regulator. The EMBO journal 23: 396-405

[2] Kawamoto, H., Koide, Y., Morita, T., and Aiba, H. (2006). Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq. Molecular microbiology 61: 1013-1022

[3] Levine, E., Zhang, Z., Kuhlman, T., and Hwa, T. (2007). Quantitative characteristics of gene regulation by small RNA. PLoS biology 5: e229