Difference between revisions of "Part:BBa K581004"
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<partinfo>BBa_K581004 short</partinfo>
 
<partinfo>BBa_K581004 short</partinfo>
  
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This is the conjugate part of the small RNA regulator sgrS2.
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'''ptsG2''' is the C87G mutant of ptsG(wt) and the conjugate part of SgrS2 in our comparator device (see Fig. 1).
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[[Image:M_P2_S2.png‎|680px]]
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PtsG is a glucose permease which is subordinate to phosphotransferase system and serves as a transporter. Here,we studied this mRNA perform the conjugate part of the small RNA regulator sgrS(wt)[1]. ptsG mRNA is regulated by SgrS by short, imperfect base-pairing interactions, and its expression is thus repressed.  
  
 
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<partinfo>BBa_K581004 parameters</partinfo>
 
<partinfo>BBa_K581004 parameters</partinfo>
 
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<h2>Background</h2>
 
<h2>Background</h2>
  
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<p>SgrS is an Hfq-binding small antisense RNA that is induced upon phosphosugar stress (Vanderpool, 2007). It forms a ribonucleoprotein complex with RNase E through Hfq to mediate silencing of the target ptsG mRNA encoding the major glucose transporter (Geissmann and Touati, 2004). A 31-nt-long stretch in the 3’ region of SgrS is partially complementary to the translation initiation region of ptsG mRNA, and a 6 nt region overlapping the Shine-Dalgarno sequence of the target mRNA turns out to be crucial for SgrS’ function, shown as Fig 2 (Kawamoto et al., 2006; Maki et al., 2010).</p>
 
  
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<p> miao </p>
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<h2>Experimental Data</h2>
 
<h2>Experimental Data</h2>

Revision as of 22:01, 5 October 2011

ptsG2-GFP (ptsG2 5'UTR fused with gfp)

ptsG2 is the C87G mutant of ptsG(wt) and the conjugate part of SgrS2 in our comparator device (see Fig. 1).

680px PtsG is a glucose permease which is subordinate to phosphotransferase system and serves as a transporter. Here,we studied this mRNA perform the conjugate part of the small RNA regulator sgrS(wt)[1]. ptsG mRNA is regulated by SgrS by short, imperfect base-pairing interactions, and its expression is thus repressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 747


This BioBrick has been sequence verified.

Background

Experimental Data

Methods

References

[1] Geissmann, T.A., and Touati, D. (2004). Hfq, a new chaperoning role: binding to messenger RNA determines access for small RNA regulator. The EMBO journal 23: 396-405

[2] Kawamoto, H., Koide, Y., Morita, T., and Aiba, H. (2006). Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq. Molecular microbiology 61: 1013-1022

[3] Levine, E., Zhang, Z., Kuhlman, T., and Hwa, T. (2007). Quantitative characteristics of gene regulation by small RNA. PLoS biology 5: e229