Difference between revisions of "Part:BBa K578002"
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===Confirmation of Part Length===
 
===Confirmation of Part Length===
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PCR of BBa_K578002 using Primers VF2 (BBa_G00100) and VFR (BBa_G00101) from pSB1C3. Note: The primers add approximatellly 200bp to the BioBrick's size.
 
[[Image:ASU_T7LRSR.png|400px]]
 
[[Image:ASU_T7LRSR.png|400px]]
  

Revision as of 21:50, 5 October 2011

T7LRSR (Listeria innocua CLIP11262)

This brick, T7LRSR, is the starting point for a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) array based around Listeria Innocua CLIP11262. The sequence of the part is composed of a T7 promoter, Lac Operator, Leader Sequence (from Listeria Innocua CLIP11262), Palindromic Repeat, Spacer (Targeted to sequence with correct protospacer and 'NGG; Protospacer-adjacent motif), followed by a palindromic repeat. After the aforementioned sequences there is a pseudospacer with the standard BioBrick suffix (with SpeI and PstI Sites). The suffix allows for parts (eg.Repeat.Spacer Repeat, RSR, formated BioBricks) to be added onto the distal end of the CRISPR array.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Confirmation of Part Length

PCR of BBa_K578002 using Primers VF2 (BBa_G00100) and VFR (BBa_G00101) from pSB1C3. Note: The primers add approximatellly 200bp to the BioBrick's size. ASU T7LRSR.png

Use of this BioBrick

This part has been [http://2011.igem.org/Team:Arizona_State/Project/L_innocua#Construction successfully ligated] into pRSF Duet along with Cas9 and tracrRNA precursor, ready for inducible CRISPR-Cas expression.