Difference between revisions of "Part:BBa K544013"
 
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The tetR-HNS2-90 protein fuses the polymerization demain of histone-like nucleoid-structuring (H-NS) protein to tetR. Thus, in E.coli, when TetR-HNS2-90 binds the TetR-specific DNA motif, it will recruit native H-NS to bind to adjacent DNA, which may lead to local gene silencing. This fusion protein is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106). Experimental data indicates that TetR-HNS2-90 is more effective than TetR on transcription repression.
 
The tetR-HNS2-90 protein fuses the polymerization demain of histone-like nucleoid-structuring (H-NS) protein to tetR. Thus, in E.coli, when TetR-HNS2-90 binds the TetR-specific DNA motif, it will recruit native H-NS to bind to adjacent DNA, which may lead to local gene silencing. This fusion protein is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106). Experimental data indicates that TetR-HNS2-90 is more effective than TetR on transcription repression.
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BBa_K544013 is one of the fusion protein that work as expected according to the analysis of experimental data as shown below.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K544013 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K544013 SequenceAndFeatures</partinfo>
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Fluorescent intensity of MG1655 -tetO2-0-sfGFP strain after transformation of various repressors
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{| style="width:254px;background:#99EE63;text-align:center;font-family: georgia, helvetica, arial, sans-serif;color:#000000;margin- top:5px;padding: 2px;" cellspacing="5";
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|[[Image:Table 1.jpg|450px]]
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|Fluorescent intensity of MG1655 -tetO2-0-sfGFP strain after transformation of various repressors
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|}
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</div>
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(The data collected are considered only when difference between values with the P-value <0.05)
 
(The data collected are considered only when difference between values with the P-value <0.05)
  
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Fluorescent intensity of MG1655 -tetO2-0-EGFP strain after transformation of various repressors
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<div ALIGN=CENTER>
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{| style="width:254px;background:#99EE63;text-align:center;font-family: georgia, helvetica, arial, sans-serif;color:#000000;margin- top:5px;padding: 2px;" cellspacing="5";
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|[[Image:Table 2.jpg|450px]]
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|Fluorescent intensity of MG1655 -tetO2-0-EGFP strain after transformation of various repressors
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|}
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</div>
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(The data collected are considered only when difference between values with the P-value <0.05)
 
(The data collected are considered only when difference between values with the P-value <0.05)
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[EGFP: Enhanced Green Fluorescent Protein, sfGFP: Superfolder Green Fluorescent protein ]
 
[EGFP: Enhanced Green Fluorescent Protein, sfGFP: Superfolder Green Fluorescent protein ]
  

Latest revision as of 19:33, 5 October 2011

tetR-HNS (2-90) fusion protein (Consitutive Promoter: BBa-J23109)

The tetR-HNS2-90 protein fuses the polymerization demain of histone-like nucleoid-structuring (H-NS) protein to tetR. Thus, in E.coli, when TetR-HNS2-90 binds the TetR-specific DNA motif, it will recruit native H-NS to bind to adjacent DNA, which may lead to local gene silencing. This fusion protein is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106). Experimental data indicates that TetR-HNS2-90 is more effective than TetR on transcription repression.

BBa_K544013 is one of the fusion protein that work as expected according to the analysis of experimental data as shown below.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 65
    Illegal SpeI site found at 37
    Illegal PstI site found at 851
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 37
    Illegal PstI site found at 851
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 65
    Illegal SpeI site found at 37
    Illegal PstI site found at 851
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 65
    Illegal SpeI site found at 37
    Illegal PstI site found at 851
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 833


Table 1.jpg
Fluorescent intensity of MG1655 -tetO2-0-sfGFP strain after transformation of various repressors


(The data collected are considered only when difference between values with the P-value <0.05)


Table 2.jpg
Fluorescent intensity of MG1655 -tetO2-0-EGFP strain after transformation of various repressors


(The data collected are considered only when difference between values with the P-value <0.05)

[EGFP: Enhanced Green Fluorescent Protein, sfGFP: Superfolder Green Fluorescent protein ]