Difference between revisions of "Part:BBa K598001:Design"
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===Source=== | ===Source=== | ||
Use mutagenesis PCR of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K598007 BBa_K598007] to alter RBS sequence. | Use mutagenesis PCR of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K598007 BBa_K598007] to alter RBS sequence. | ||
| + | Engineered RBS is selected from constitutive library based on RBS calculator. | ||
===References=== | ===References=== | ||
Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004) A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research, 32, 1610-1614. | Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004) A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research, 32, 1610-1614. | ||
Revision as of 19:25, 5 October 2011
Theophylline Responsive Riboswitch 1G1 with Engineered RBS+GFP generator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 71
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 730
Design Notes
The RBS of this device is altered into AGGAGGU. When changing the RBS, the anti-RBS sequence should be changed as well. Theophylline riboswich is composed of a synthesized theophylline aptamer and a regulatory platform.
Source
Use mutagenesis PCR of BBa_K598007 to alter RBS sequence. Engineered RBS is selected from constitutive library based on RBS calculator.
References
Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004) A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research, 32, 1610-1614.