Difference between revisions of "Part:BBa K598001:Design"
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===Design Notes===
 
===Design Notes===
 
The RBS of this device is altered into AGGAGGU. When changing the RBS, the anti-RBS sequence should be changed as well.
 
The RBS of this device is altered into AGGAGGU. When changing the RBS, the anti-RBS sequence should be changed as well.
 +
Theophylline riboswich is composed of a synthesized theophylline aptamer and a regulatory platform.
  
  
  
 
===Source===
 
===Source===
−
Mutagenesis PCR  
+
Use mutagenesis PCR of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K598007 BBa_K598007] to alter RBS sequence.
−
<p><b>For the full characterisation of the device, please refer to [https://parts.igem.org/wiki/index.php?title=Part:BBa_K598002 BBa_K598002]
+
−
Theophylline riboswich is composed of a synthesized theophylline aptamer and a regulatory platform.
+
  
 
===References===
 
===References===
 
Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004) A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research, 32, 1610-1614.
 
Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004) A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research, 32, 1610-1614.

Revision as of 19:22, 5 October 2011

Theophylline Responsive Riboswitch 1G1 with Engineered RBS+GFP generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 71
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 730


Design Notes

The RBS of this device is altered into AGGAGGU. When changing the RBS, the anti-RBS sequence should be changed as well. Theophylline riboswich is composed of a synthesized theophylline aptamer and a regulatory platform.


Source

Use mutagenesis PCR of BBa_K598007 to alter RBS sequence.

References

Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004) A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research, 32, 1610-1614.